Hydrolases of pulmonary lysosomes and lamellar bodies.
نویسندگان
چکیده
منابع مشابه
Hydrolases of pulmonary lysosomes and lamellar bodies.
Lamellar bodies and lysosomes from the lungs of rabbits were examined for the presence of 24 hydrolytic enzymes. All hydrolases detectable in lysosomes were also found in lamellar bodies. Hydrolase activities in lamellar bodies were not due to contamination with lysosomes as shown by isopycnic centrifugation of the subcellular organelles on continuous density sucrose gradients. Lysosomal prepar...
متن کاملSurface-expressed lamellar body membrane is recycled to lamellar bodies.
Monoclonal antibody (MAb) 3C9, an antibody generated to the lamellar body of rat lung type II pneumocytes, specifically labels the luminal face of the lamellar body membrane. To follow the retrieval of lamellar body membrane from the cell surface in these cells, MAb 3C9 was instilled into rat lungs. In vivo, it was endocytosed by type II cells but not by other lung cells. In type II cells that ...
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BACKGROUND/AIM Although lamellar bodies have been the center of interest over the last years, the published results of fetal pulmonary maturity determination according to their concentration in amniotic fluid are controversial. The aim of this study was to determine the significance of lamellar bodies, as well as the ratio lecithin/sphingomyelin (L/S) in amniotic fluid for the assessment of fet...
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Type II cells of the alveolar epithelium of adult rats have been shown to internalize the ferritin-labeled lectin from Maclura pomifera (MPA-F). This alpha-galactose-binding molecule binds specifically to the apical plasma membrane of the cells. Once within the cell the lectin is cycled from pinocytic vesicles, to multivesicular bodies of two types, and finally to lamellar bodies, the storage g...
متن کاملSurfactant protein composition of lamellar bodies isolated from rat lung.
Lamellar bodies isolated from rat lung contain all three classes of surfactant proteins, SP-A, SP-B and SP-C, as determined by immunoblot analysis. The amounts of the surfactant proteins present in lamellar bodies, determined by sandwich e.l.i.s.a. (SP-A) and fluorescamine assay (SP-B and SP-C) show that these organelles are highly enriched in the hydrophobic surfactant proteins SP-B and SP-C.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34262-5